primary antibodies tert  (Novus Biologicals)

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: (a) PCR-amplified products of TERT promoter from conjunctival SCC cases (M, DNA size marker). (b) Schematic representation showing the translation (ATG) start site, transcription start site, and known hotspot mutations in TERT promoter (the region highlighted in red) (c) Wild-type chromatogram of the promoter region of TERT from normal skin obtained by Sanger sequencing. (d and e) Sequence chromatograms showing the wild-type sequence. (f and g) The mutations c.-146C>T (C250T; Chr.5: 1295250C>T) and c.-124C>T (C228T; Chr.5:1295228C>T) identified in TERT promoter locus in conjunctival SCC cases

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Amplification, Marker, Sequencing

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: Immunohistochemistry scoring

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Immunohistochemistry, Staining

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: (a) Strong cytoplasmic positivity of TERT in a case of conjunctival squamous cell carcinoma. (b) Positive control showing cytoplasmic positivity of TERT in a case of conjunctival melanoma tissue (c) Negative control; primary antibody replaced by TBS. (d) low expression of TERT in skin eyelid

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Positive Control, Negative Control, Expressing

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: TERT immunohistochemical expression and association with clinicopathologic features and TERT promoter mutation in ocular surface squamous cell carcinoma

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Immunohistochemical staining, Expressing, Mutagenesis

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: Kaplan–Meier analysis showing reduced disease-free survival rates in conjunctival squamous cell carcinoma in patients with positive TERT expression

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Expressing

Journal: Indian Journal of Ophthalmology

Article Title: Immunohistochemical and mutational status of telomerase reverse transcriptase in conjunctival squamous cell carcinoma

doi: 10.4103/ijo.IJO_1342_21

Figure Lengend Snippet: TERT promoter mutation and association with clinicopathological features in ocular surface squamous cell carcinoma

Article Snippet: After cooling, the slides were washed using TBS (pH 7.5) and then incubated for 20 min with 0.3%-v/v H 2 O 2 , followed by incubation with primary monoclonal antibodies against human TERT (A-6, SCBT, Santa Cruz, CA, USA) at a dilution of 1:50 and were processed using the UltravisionQuanto detection system (Thermo Scientific, Fermont CA, USA).

Techniques: Mutagenesis